polyclonal goat igg antibody against caiv  (R&D Systems)

Journal: Cells, Tissues, Organs

Article Title: Carbonic Anhydrase IV Deficiency Causes Intrauterine Embryonic Loss in Mice

doi: 10.1159/000544000

Figure Lengend Snippet: CAIV immunostaining of reproductive organs in nonpregnant mice. a–d Ovary. No immunostaining is visible in the mullerian epithelium (ME in a ), ovarian stroma (S in a ), primordial follicles (arrow in a ), primary follicles (* in b ), secondary follicles (SF in b ), tertiary follicles ( c ), and corpora lutea ( d ). e , f Fallopian tube. Neither in the ampulla ( e ) nor in the isthmus ( f ) is CAIV immunostaining observed in the epithelium (Ep) and musculature (M). In the uterus ( g ), endometrial capillaries were CAIV immunoreactive (insert in g ), while no staining is visible in the endometrial epithelium (Ep) and the endometrial stromal cells (S). h Vagina. In the vaginal tissue, no CAIV-immunoreactive cells are observed in the epithelium (Ep) or in the stroma (S). i In kidney as positive control, a strong CAIV signal is visible in tubular epithelium while the negative control of the endometrium ( j ) shows no immunostaining (counterstaining with hematoxylin). Bar, 50 μm.

Article Snippet: After rinsing in distilled water, slices were blocked with PBS-glucose-oxidase buffer and incubated with a polyclonal goat IgG antibody against CAIV (AF2414; R&D Systems, Minneapolis, MN, USA) (1:100 in PBS/5% BSA) overnight at 4°C, followed by incubation with biotinylated anti-goat IgG (1:200 in PBS/5% BSA) for 30 min at room temperature.

Techniques: Immunostaining, Staining, Positive Control, Negative Control

Journal: Cells, Tissues, Organs

Article Title: Carbonic Anhydrase IV Deficiency Causes Intrauterine Embryonic Loss in Mice

doi: 10.1159/000544000

Figure Lengend Snippet: CAIV immunostaining of mouse embryos at developmental stages 4.5 ( a ), 5.5 ( b ), 6.5 ( c ), and 7.5 dpc ( d ). CAIV immunoreactivity is visible in the blastocyst (B), the visceral endoderm (VE), and the embryonic ectoderm (IE). Corresponding negative controls ( a ′, b ′, c ′, d ′) show no immunostaining. Slides are counterstained with hematoxylin. Bar, 50 μm in a , b , 100 μm in c , d , 100 μm in a ′, b ′, and 160 μm in c ′, d ′.

Article Snippet: After rinsing in distilled water, slices were blocked with PBS-glucose-oxidase buffer and incubated with a polyclonal goat IgG antibody against CAIV (AF2414; R&D Systems, Minneapolis, MN, USA) (1:100 in PBS/5% BSA) overnight at 4°C, followed by incubation with biotinylated anti-goat IgG (1:200 in PBS/5% BSA) for 30 min at room temperature.

Techniques: Immunostaining

Journal: Cells, Tissues, Organs

Article Title: Carbonic Anhydrase IV Deficiency Causes Intrauterine Embryonic Loss in Mice

doi: 10.1159/000544000

Figure Lengend Snippet: CAIV immunostaining of a representative mouse embryo at 8.5 dpc in overview ( a ) and in higher magnification ( b , c ). A strong immunoreactivity is visible in the amniotic membrane (AM), the yolk sac epithelium (Y), trophoblast giant cells (TGC), the endometrial epithelium (EE), and the developing GT of the embryo (E). Corresponding negative controls ( a ′, b ′, c ′) show no immunostaining. Slides are counterstained with hematoxylin. Bar indicates 200 μm in a and 50 μm in b , c .

Article Snippet: After rinsing in distilled water, slices were blocked with PBS-glucose-oxidase buffer and incubated with a polyclonal goat IgG antibody against CAIV (AF2414; R&D Systems, Minneapolis, MN, USA) (1:100 in PBS/5% BSA) overnight at 4°C, followed by incubation with biotinylated anti-goat IgG (1:200 in PBS/5% BSA) for 30 min at room temperature.

Techniques: Immunostaining, Membrane

Journal: Cells, Tissues, Organs

Article Title: Carbonic Anhydrase IV Deficiency Causes Intrauterine Embryonic Loss in Mice

doi: 10.1159/000544000

Figure Lengend Snippet: CAIV immunostaining of mouse embryos at 9.5 dpc in overview ( a , b ) and in higher magnification ( c–h ). Within the developing embryo ( c , d ), CAIV immunostaining is visible in the gut tube (GT), the floor plate of the neural tube (FP), and the notochord (N). In placenta and extraembryonic tissues ( e–h ), the labyrinthine compartment (LT), the endometrial epithelium (EE), the trophoblast giant cells (TGC), and the yolk sac epithelium (Y) show immunoreactivity. The corresponding negative control ( b ) shows no immunostaining (counterstaining with hematoxylin). Bar = 500 μm in a , b and 50 μm in c–h .

Article Snippet: After rinsing in distilled water, slices were blocked with PBS-glucose-oxidase buffer and incubated with a polyclonal goat IgG antibody against CAIV (AF2414; R&D Systems, Minneapolis, MN, USA) (1:100 in PBS/5% BSA) overnight at 4°C, followed by incubation with biotinylated anti-goat IgG (1:200 in PBS/5% BSA) for 30 min at room temperature.

Techniques: Immunostaining, Negative Control

Journal: Cells, Tissues, Organs

Article Title: Carbonic Anhydrase IV Deficiency Causes Intrauterine Embryonic Loss in Mice

doi: 10.1159/000544000

Figure Lengend Snippet: Litter size and sex distribution in heterozygous and CAIV knockout mating. a Mating of CAIV knockout mice ( n = 17 litters) results in a significant reduction of litter size compared to heterozygous mating ( n = 31 litters). b Homozygous CAIV knockout mating results in a non-mendalian sex distribution of the 54 CAIV knockout pubs analyzed (74% male and 26% female instead of 50% each). c In heterozygous mating, the 164 pups analyzed show an enhanced number of wild-type (31% instead of 25% expected) and heterozygous genotype (62% instead of 50% expected), whereas the number of CAIV knockout pups is significantly reduced (7% instead of 25% expected). d A non-mendelian sex distribution is also observed in the 12 CAIV knockout pups (shown in c ) resulting from heterozygous mating (67% male and 33% female instead of 50% each), while there is a slight preponderance of female pups in both the 50 resulting wild-type (36% male vs. 64% female) and the 102 resulting CAIV heterozygous pups (46% male vs. 54% female). e On 9.5 dpc, number of implantation chambers is similar in heterozygous ( n = 3) and CAIV knockout ( n = 5) mating. The sex distribution of embryos is slightly shifted to female in both experimental groups, showing a higher percentage of female embryos in heterozygous mating in total ( n = 26; 40% male vs. 60% female) as well as in CAIV knockout embryos resulting from heterozygous mating (40% male vs. 60% female). f Additionally, CAIV knockout mating results in a higher percentage of female embryos on day 9.5 of pregnancy ( n = 21; 43% male vs. 57% female).

Article Snippet: After rinsing in distilled water, slices were blocked with PBS-glucose-oxidase buffer and incubated with a polyclonal goat IgG antibody against CAIV (AF2414; R&D Systems, Minneapolis, MN, USA) (1:100 in PBS/5% BSA) overnight at 4°C, followed by incubation with biotinylated anti-goat IgG (1:200 in PBS/5% BSA) for 30 min at room temperature.

Techniques: Knock-Out